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. 2013 Apr 3;4(4):259–265. doi: 10.1007/s13238-013-2114-1

Pannexin-1 influences peritoneal cavity cell population but is not involved in NLRP3 inflammasome activation

Hongbin Wang 12114, Yue Xing 12114, Liming Mao 12114, Yi Luo 22114, Lishan Kang 22114, Guangxun Meng 12114,✉
PMCID: PMC4875518  PMID: 23549611

Abstract

Pannexin-1 (Panx1) forms nonselective large channel in cell plasma membrane and has been shown to be associated with NLRP3 inflammasome activation, ATP release and phagocytes recruitment. In the current study, by manipulation of Panx1 expression in human myeloid cells and application of Panx1 deficient mice, we failed to find a correlation between Panx1 and NLRP3 inflammasome activation, although an interaction between these two proteins was evident. However, in thioglycollate induced peritonitis, Panx1 deficient mice showed much more phagocytes infiltration. Further analyses showed that mice deficient for Panx1 exhibited enlarged F4/80lowGr1−Ly6C−cell population in the peritonea. Our study thus reveals an important role for Panx1 in regulation of peritoneal cell population and peritonitis development.

Electronic Supplementary Material

Supplementary material is available for this article at 10.1007/s13238-013-2114-1 and is accessible for authorized users.

Keywords: innate Immunity, inflammasome, NLRP3, Pannexin-1, peritonitis

Electronic supplementary material

13238_2013_2114_MOESM1_ESM.pdf (1.3MB, pdf)

Supplementary material, approximately 1.27 MB.

Footnotes

Electronic Supplementary Material

Supplementary material is available for this article at 10.1007/s13238-013-2114-1 and is accessible for authorized users.

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Supplementary Materials

13238_2013_2114_MOESM1_ESM.pdf (1.3MB, pdf)

Supplementary material, approximately 1.27 MB.

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