Table 1.
List of primary antibodies used in this study.
| Primary antibody | Manufacturer | Dilution |
|---|---|---|
| Insulin receptor (InsR) | Santa Cruz, sc-711 | 1/400 |
| Insulin like growth factor receptor type 1 IGF1R | Santa Cruz, sc-713 | 1/400 |
| Catalytic subunits of phosphoinositide 3-kinase (p110α/β) | Cell Signalling, 4249, 3011 | 1/1000 |
| Regulatory subunits of phosphoinositide 3-kinase (p85α) | Millipore, 06-195 | 1/5000 in 1% milk |
| Kinase Akt | Cell Signalling, 9272 | 1/1000 |
| Phosphorylated (p)Akt Thr308 | Cell Signalling, 9275 | 1/1000 |
| pAkt Ser473 | Cell Signalling, 9271 | 1/1000 |
| Glycogen synthase kinase 3 (GSK3) | Cell Signalling, 9315 | 1/1000 |
| pGSK3 Ser21/9 | Cell Signalling, 9331 | 1/1000 |
| Ribosomal S6 kinase (S6K) | Cell Signalling, 2708 | 1/1000 |
| pS6K Thr 389 | Cell Signalling, 9234 | 1/1000 |
| Eukaryotic translocation initiation factor 4 binding protein (4EBP) | Cell Signalling, 9644 | 1/1000 |
| p4EBP Ser65 | Cell Signalling, 9451 | 1/1000 |
| Mitogen activated protein kinase (MAPK) | Cell Signalling, 4695 | 1/1000 |
| pMAPK Thr202/Tyr204 | Cell Signalling, 4370 | 1/1000 |
| Lipoprotein lipase (LPL) | Abcam, 21356 | 1/1000 |
| Sterol regulatory element binding protein (SREBP) | Abcam, 3259 | 1/200 |
| Fatty acid synthase (FAS) | Cell Signalling, 3180 | 1/1000 |
| Peroxisome proliferator – activated receptor alpha (PPARα) | Abcam, 8934 | 1/1500 |
| Peroxisome proliferator – activated receptor gamma (PPARγ) | Santa Cruz, sc-7273 | 1/200 |
| Fatty acid transport protein 1 (FATP1) | Santa Cruz, sc-31955 | 1/400 |