S1 Fig is incorrect. Please view the correct S1 Fig below.
There are errors in S1 and S2 Tables. The pET30a(+) vector of both tables should read pET41a(+).
Supporting Information
Bacteria were cultured in LB medium at 37°C to OD600 0.5. The recombinant proteins were induced for 3 h by supplementation with 0.5 mM IPTG. Cells were harvested, washed in PBS, and then resuspended in 10 mM PBS (pH7.5, 500 mM NaCl). After several freeze/thaw cycles, the cell suspension was sonicated for 3 min with an interval of 4 s between pulses, and then centrifuged at 5000 g for 10 min at 37°C. Twenty microliter supernatant samples were analysed by 12% SDS-PAGE.
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Reference
- 1.Xia T, Li Y, Sun D, Zhuo T, Fan X, Zou H (2016) Identification of an Extracellular Endoglucanase That Is Required for Full Virulence in Xanthomonas citrisubsp. citri. PLoS ONE 11(3): e0151017 doi: 10.1371/journal.pone.0151017 [DOI] [PMC free article] [PubMed] [Google Scholar]
Associated Data
This section collects any data citations, data availability statements, or supplementary materials included in this article.
Supplementary Materials
Bacteria were cultured in LB medium at 37°C to OD600 0.5. The recombinant proteins were induced for 3 h by supplementation with 0.5 mM IPTG. Cells were harvested, washed in PBS, and then resuspended in 10 mM PBS (pH7.5, 500 mM NaCl). After several freeze/thaw cycles, the cell suspension was sonicated for 3 min with an interval of 4 s between pulses, and then centrifuged at 5000 g for 10 min at 37°C. Twenty microliter supernatant samples were analysed by 12% SDS-PAGE.
(TIF)
(DOCX)
(DOCX)