Figure 1.

Suppression of T cell proliferation and activation by BMX-001 PBMCs from pigs (n = 4) and humans (n = 3) were stimulated with PHA for 72h with/without 10µM BMX-001. BMX-001 significantly reduced proliferation of both pig (A) and human (D) PBMCs stimulated with PHA (*p<0.01). Results are expressed as ³H-thymidine incorporation (counts per minute). The percentages of T cells in pigs (B,C) and humans (E,F) secreting the inflammatory cytokines, IFN-γ or TNF-α, were measured after intracellular staining of whole blood. In the presence of 10µM BMX-001, the percentages of both IFN-γ- or TNF-α-secreting CD4⁺ and CD8⁺ T cells were significantly decreased in humans (*p<0.01) whereas only the percentage of IFN-γ secreting CD8⁺ T cells was significantly decreased in pigs (*p<0.05). Data are expressed as mean ± SEM.