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. 1992 Apr 1;89(7):3088–3092. doi: 10.1073/pnas.89.7.3088

Transactivation and transformation by Myb are negatively regulated by a leucine-zipper structure.

C Kanei-Ishii 1, E M MacMillan 1, T Nomura 1, A Sarai 1, R G Ramsay 1, S Aimoto 1, S Ishii 1, T J Gonda 1
PMCID: PMC48809  PMID: 1557416

Abstract

The negative regulatory domain of the c-myb protooncogene product (c-Myb) normally represses transcriptional activation by c-Myb. We show here that a leucine-zipper structure is a component of the negative regulatory domain, because its disruption markedly increases both the transactivating and transforming capacities of c-Myb. We also demonstrate that this leucine-zipper structure can interact with cellular proteins. Our results suggest that an inhibitor that suppresses transactivation binds to c-Myb through the leucine zipper and that c-Myb can be oncogenically activated by missense mutation.

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