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. 1992 Apr 15;89(8):3175–3179. doi: 10.1073/pnas.89.8.3175

Human combinatorial antibody libraries to hepatitis B surface antigen.

S L Zebedee 1, C F Barbas 3rd 1, Y L Hom 1, R H Caothien 1, R Graff 1, J DeGraw 1, J Pyati 1, R LaPolla 1, D R Burton 1, R A Lerner 1, et al.
PMCID: PMC48828  PMID: 1373487

Abstract

Human antibody Fab fragments that bind to hepatitis B surface antigen (HBsAg) were generated by using a recombinant phage surface-display expression system. Characterization of HBsAg-specific Fab fragments isolated from two vaccinated individuals reveals diversity in specificity of antigen binding and in the sequences of the complementarity-determining region. The sequence results show examples of human light-chain promiscuity that result in fine specificity changes and a strong relationship to a human germ-line gene. This application illustrates further that this technique is a powerful tool to isolate distinct human antibodies against immunogenic viral targets.

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Selected References

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