Skip to main content
. 2016 Jan 8;7(7):7801–7815. doi: 10.18632/oncotarget.6848

Figure 7. PHLPP regulates integrin protein expression.

Figure 7

A. Cell lysates prepared from PHLPP overexpressing Panc-1 and PHLPP knockdown ASPC-1 cells were analyzed for the expression of β1 and β4 integrin and tubulin using immunoblotting. B–C. Relative expression of β1 (B) and β4 integrin (C) was calculated and normalized to tubulin. The level in control cells was set to 1. Data represent the mean ± SEM (n = 3, * p<0.05 by two-sample t-tests). D–E. Total RNAs were isolated from PHLPP overexpressing Panc-1 cells (D) and PHLPP knockdown ASPC-1 cells (E). Real-time PCR analysis was performed using probes specific for human β1 and β4 integrin genes. Each experimental point was done in triplicates, and the graphs represent the mean ± SD (n = 3).