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. 2016 Jan 22;7(7):8043–8054. doi: 10.18632/oncotarget.6976

Figure 1. Lentivirus-mediated PLCγ1 shRNA could block proliferation in human gastric adenocarcinoma BGC-823 cells.

Figure 1

BGC-823 cell line of stable expressing PLCγ1shRNA was established with the transduction of four types PLCγ1shRNAs using a lentiviral transduction strategy. (A) The effect of PLCγ1shRNAs on the level of PLCγ1 protein was detected with Western blotting analysis as described in Materials and Methods. (B) The effect of PLCγ1shRNAs on cell growth rate was measured with MTT assay as described in Materials and Methods. (C) The effect of PLCγ1 shRNA2/3 on cloning formation was detected with Colony formation assay as described in Materials and Methods. (D) The levels of PCNA, cleaved-PARP, PARP, Bcl-2, PLCγ1, and GAPDH protein were detected with Western blotting analysis as described in Materials and Methods. Data are reported as means ± S.D. of three independent experiments (**P < 0.01, ***P < 0.01, ****P < 0.0001, vs respective control).