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. 2016 May 2;8(5):108. doi: 10.3390/v8050108

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© 2016 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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Puumala virus (PUUV) L protein possesses a functional endonuclease domain. (A) Sequence alignment of the N-terminal domains of HTNV and PUUV L protein. (B) BSR T7/5 cells were transfected with wt or D97A PUUV and HTNV L protein N-terminal constructs along with an NLuc reporter control plasmid. Cells were lyzed 48 hours post-transfection. Proteins were separated by SDS-PAGE and transferred on membranes for immunoblotting. (C) NLuc activity was measured using the Nano Glow^® Luciferase Assay System (Promega). Data are expressed as mean ± SD (n = 3) and were analyzed by one-way ANOVA with p-values indicated.