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. 2016 May 21;8(5):134. doi: 10.3390/v8050134

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© 2016 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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Scheme of the insertion sites in the VACV genome. The diagram of the VACV genome with the HindIII restriction sites is shown, including the location of the different insertion sites. BamHI: BamHI site of the HindIII F fragment; HA: hemagglutinin gene; VGF: VACV growth factor gene; N2: N2 gene; M1: M1 gene encodes the large subunit of ribonucleotide reductase (RR); TK: thymidine kinase gene; A27L: gene that encodes the 14 kDa fusion protein; ITRs: inverted terminal repeats. Adapted from [13].