Skip to main content
NCBI home page
Journal of Neurology, Neurosurgery, and Psychiatry logoLink to Journal of Neurology, Neurosurgery, and Psychiatry
. 1991 May;54(5):412–414. doi: 10.1136/jnnp.54.5.412

Immunoglobulin producing cells in bone marrow and blood of patients with multiple sclerosis and controls.

S Fredrikson 1, S Baig 1, H Link 1
PMCID: PMC488539  PMID: 1713951

Abstract

Multiple sclerosis (MS) is characterised by intrathecal synthesis of IgG, less frequently of IgA and IgM. Local production of antibodies to myelin basic protein (MBP) and other myelin components has also been reported, and autoimmune pathogenesis has been postulated. Whether MS is accompanied by a systemic B cell response is less clear. To elucidate this question, we examined bone marrow and peripheral blood from patients with MS and controls for cells secreting IgG, IgA and IgM, as well as anti-MBP antibodies of these three isotypes. Patients with MS without any signs of concurrent infections had higher numbers of IgG + IgA + IgM secreting cells both in bone marrow and peripheral blood compared with healthy controls. The same abnormalities were observed in patients with other inflammatory neurological diseases (OIND). When analysing individual isotypes, patients with MS and OIND had higher numbers of IgA secreting cells both in bone marrow and blood compared with healthy controls. Only one of 13 MS patients examined had anti-MBP antibody secreting cells in bone marrow and blood. The systemic B cell response registered in MS is also present in other inflammatory neurological diseases and its specificity and possible role in the pathogenesis of MS remains unknown.

Full text

PDF
412

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Benner R., Hijmans W., Haaijman J. J. The bone marrow: the major source of serum immunoglobulins, but still a neglected site of antibody formation. Clin Exp Immunol. 1981 Oct;46(1):1–8. [PMC free article] [PubMed] [Google Scholar]
  2. Czerkinsky C. C., Nilsson L. A., Nygren H., Ouchterlony O., Tarkowski A. A solid-phase enzyme-linked immunospot (ELISPOT) assay for enumeration of specific antibody-secreting cells. J Immunol Methods. 1983 Dec 16;65(1-2):109–121. doi: 10.1016/0022-1759(83)90308-3. [DOI] [PubMed] [Google Scholar]
  3. Deibler G. E., Martenson R. E., Kies M. W. Large scale preparation of myelin basic protein from central nervous tissue of several mammalian species. Prep Biochem. 1972;2(2):139–165. doi: 10.1080/00327487208061467. [DOI] [PubMed] [Google Scholar]
  4. Elsevier S. M., Ruddle F. H. Haploid genome reactivation and recovery by cell hybridization. Induction of DNA synthesis in spermatid nuclei. Chromosoma. 1976 Jul 8;56(3):227–241. doi: 10.1007/BF00293187. [DOI] [PubMed] [Google Scholar]
  5. Fauci A. S., Moutsopoulos H. M. Polyclonally triggered B cells in the peripheral blood and bone marrow of normal individuals and in patients with systemic lupus erythematosus and primary Sjögren's syndrome. Arthritis Rheum. 1981 Apr;24(4):577–583. doi: 10.1002/art.1780240402. [DOI] [PubMed] [Google Scholar]
  6. Fredrikson S., Kam-Hansen S., Lu C. Z., Eriksson L. C. Bone marrow cells in patients with multiple sclerosis. J Neuroimmunol. 1989 Sep;24(1-2):23–31. doi: 10.1016/0165-5728(89)90094-5. [DOI] [PubMed] [Google Scholar]
  7. Henriksson A., Kam-Hansen S., Link H. IgM, IgA and IgG producing cells in cerebrospinal fluid and peripheral blood in multiple sclerosis. Clin Exp Immunol. 1985 Oct;62(1):176–184. [PMC free article] [PubMed] [Google Scholar]
  8. Kutteh W. H., Prince S. J., Mestecky J. Tissue origins of human polymeric and monomeric IgA. J Immunol. 1982 Feb;128(2):990–995. [PubMed] [Google Scholar]
  9. Laurenzi M. A., Mavra M., Kam-Hansen S., Link H. Oligoclonal IgG and free chains in multiple sclerosis demonstrated by thin-layer polyacrylamide gel isoelectric focusing and immunofixation. Ann Neurol. 1980 Sep;8(3):241–247. doi: 10.1002/ana.410080304. [DOI] [PubMed] [Google Scholar]
  10. Link H., Baig S., Jiang Y. P., Olsson O., Höjeberg B., Kostulas V., Olsson T. B cells and antibodies in MS. Res Immunol. 1989 Feb;140(2):219–248. doi: 10.1016/0923-2494(89)90091-6. [DOI] [PubMed] [Google Scholar]
  11. McMillan R., Longmire R. L., Yelenosky R., Lang J. E., Heath V., Craddock C. G. Immunoglobulin synthesis by human lymphoid tissues: normal bone marrow as a major site of IgG production. J Immunol. 1972 Dec;109(6):1386–1394. [PubMed] [Google Scholar]
  12. Möller S. A., Borrebaeck C. A. A filter immuno-plaque assay for the detection of antibody-secreting cells in vitro. J Immunol Methods. 1985 May 23;79(2):195–204. doi: 10.1016/0022-1759(85)90099-7. [DOI] [PubMed] [Google Scholar]
  13. Sedgwick J. D., Holt P. G. A solid-phase immunoenzymatic technique for the enumeration of specific antibody-secreting cells. J Immunol Methods. 1983 Feb 25;57(1-3):301–309. doi: 10.1016/0022-1759(83)90091-1. [DOI] [PubMed] [Google Scholar]
  14. Tarkowski A., Klareskog L., Carlsten H., Herberts P., Koopman W. J. Secretion of antibodies to types I and II collagen by synovial tissue cells in patients with rheumatoid arthritis. Arthritis Rheum. 1989 Sep;32(9):1087–1092. doi: 10.1002/anr.1780320906. [DOI] [PubMed] [Google Scholar]
  15. Vaughan J. H., Chihara T., Moore T. L., Robbins D. L., Tanimoto K., Johnson J. S., McMillan R. Rheumatoid factor-producing cells detected by direct hemolytic plaque assay. J Clin Invest. 1976 Oct;58(4):933–941. doi: 10.1172/JCI108546. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Zachau A. C., Strigård K., Baig S., Höjeberg B., Olsson T. Distribution of plasma cells secreting antibodies against nervous tissue antigens during experimental allergic encephalomyelitis enumerated by a nitrocellulose immunospot assay. J Neurol Sci. 1989 Jul;91(3):323–336. doi: 10.1016/0022-510x(89)90061-0. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Neurology, Neurosurgery, and Psychiatry are provided here courtesy of BMJ Publishing Group

RESOURCES