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. 2016 Apr 15;18(5):265–272. doi: 10.1016/j.neo.2016.03.005

Figure 1.

Figure 1

Expression levels and knockdown effects of GALNT6 in pancreatic cancer cells. (A) Expression levels of endogenous GALNT6 and Mucin 4 proteins in 14 pancreatic cancer cell lines were examined by Western blot analysis. (B) The expression levels of GALNT6 and MUC4 were successfully downregulated in KLM-1 and Panc02.03 cells 72 hours after transfection with si-GALNT6 compared with si-control. (C) GALNT6 and Mucin 4 protein levels were reduced in KLM-1 and Panc02.03 cells 72 hours after transfection with si-GALNT6 compared with si-control. (D) The transcriptional level of MUC4 was downregulated by GALNT6 knockdown. (E) Cell viability of KLM-1 and Panc02.03 cells was assessed by MTT assays 7 days after transfection with si-GALNT6 or si-control. Asterisks indicate the statistical significance with P value of < .001.