Figure 3.

Suppression of Mucin 4 signal pathways by GALNT6 knockdown. (A) Silencing of GALNT6 expression diminished Mucin 4 protein and its downstream molecules HER2, ERK, and P-cadherin proteins, which was accompanied by increase in E-cadherin protein level. (B) Seventy-two hours after treatment with si-control or si-GALNT6, microscopic observation was conducted to monitor cellular morphological changes. (C) Cell morphology was further investigated by immunostaining with a fluorescence-labeled phalloidin (green) at 4 days after transfection with si-control (top) or si-GALNT6 (bottom). Scale bars indicate 10 μm.