Video 5. Vacuoles in Fig4-/- OLs appear and disappear within hours.
Time-lapse live cell analysis of Fig4-/- primary OPC/OLs imaged with an IncuCyte ZOOM microscope. Phase contrast images were taken every 2 hr over a time interval of 60 hr. The majority of Fig4-/- cells contain large perinuclear vacuoles. Some of these vacuoles appear and disappear within hours (n = 3). Scale bar = 60 µm.