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. 2016 Feb 1;7(9):9680–9691. doi: 10.18632/oncotarget.7109

Figure 3. EGFRvIII–stimulated GBP1 expression is p38 MAPK dependent.

Figure 3

A. After 24 h of serum starvation, U87-EGFRvIII cells were treated with DMSO (−), 10 μM of the EGFR tyrosine kinase inhibitor AG1478 (AG), or 20μM of the p38 inhibitor SB203580 (SB) for an additional 24 h before Western blot analysis. B. U87-EGFRvIII cells were transfected with the indicated concentration of p38 siRNA (si-p38) or control siRNA (si-Luc) for 24 h and then serum starved for 24 h followed by Western blot analysis. The p38 siRNAs were described previously [10]. C. U87-EGFRvIII cells were transfected with pGL3-237 and pRL-TK for 24 h and then serum starved for 24 h. The starved cells were pretreated with DMSO or 20 μM SB203580 for an additional 24 h before reporter assay. This result is expressed as the mean of three independent experiments ± SD. *, P < 0.01. D. Levels of GBP1, EGFRvIII and phospho-p38 were assessed by Western blot analysis in U87 cells expressing low, medium and high levels of EGFRvIII, which were generated as described elsewhere [25].