Table 1. Clinical characteristics of the patients with breast cancer.

Samples		HER2 positive (IHC + + + and + + with gene amplification by FISH)	HER2 negative (IHC−, +, and ++ without gene amplification by FISH)	Normal control	p
Sample No.		36	60	79
Age(year)		52.3(31~82)	53.1(31~78)	46.4(24~86)	>0.05
ER positive		n=11 (5 for test set) as Luminal B subtype	n=42 (22 for test set) as Luminal A subtype	\
ER negative		n=25 (10 for test set) as HER2-enriched subtype	n=18 (8 for test set) as Basal-like subtype	\
TNM classification stage	I	6	25	\
	IIA	19	20	\
	IIIA	1	1	\
	IIIB	10	14	\

HER2, human epidermal growth factor receptor 2;

ER, estrogen receptor;

IHC, immunohistochemistry;

FISH, fluorescence in situ hybridization.