Figure 3. miR-217 negatively regulates the expression of CAGE.

(A) QRT-PCR analysis employing the indicated cancer cells was performed to determine the expression of CAGE and miR-217 in the indicated cancer cells. Immunoblot analysis was also performed. *p < 0.05; **p < 0.005; ***p < 0.0005. (B) The indicated cancer cells were transiently transfected with various concentrations of miR-217 construct. At 48 h after transfection, cell lysates were subjected to immunoblot analysis. (C) SNU387^R-miR-217 or Malme3M^R-miR-217 cells were transiently transfected with control inhibitor (10 nM) or miR-217 inhibitor (10 nM). At 48 h after transfection, cell lysates were subjected to immunoblot analysis. Cell lysates isolated from SNU387/SNU387^R and Malme3M/Malme3M^R cells were also subjected to immunoblot analysis. (D) Malme3M cells were transiently transfected with the control inhibitor (10 nM) or miR-217 inhibitor (10 nM). At 48 h after transfection, qRT-PCR analysis was performed to determine the expression of miR-217 and CAGE (left panel). Cell lysates were also subjected to immunoblot analysis (right panel). *p < 0.05; **p < 0.005.