Figure 2.

Gene expression profile of GATA4-deficient TM4 cells. Microarray analysis (n = 3 per group) was performed using the Illumina Mouse WG-6 v2.0 BeadChip. After background correction, quantile normalization, and log2 transformation, differentially expressed genes were identified using LIMMA with the Benjamini-Hochberg correction. Only probes with expression levels with at least 1.5-fold difference and a FDR below 5% were considered significantly differentially expressed. A heatmap showing the top 50 DEGs (sorted according to their log2 fold changes) was generated with R. Red represents down-regulation and green signifies up-regulation of the particular probes.