Abstract
Zooxanthellae are unicellular algae that occur as endosymbionts in many hundreds of common marine invertebrates. The issue of zooxanthella diversity has been difficult to address. Most zooxanthellae have been placed in the dinoflagellate genus Symbiodinium as one or several species that are not easily distinguished. We compared Symbiodinium and nonsymbiotic dinoflagellates using small ribosomal subunit RNA sequences. Surprisingly, small ribosomal subunit RNA diversity within the genus Symbiodinium is comparable to that observed among different orders of nonsymbiotic dinoflagellates. These data reinforce the conclusion that Symbiodinium-like zooxanthellae represent a collection of distinct species and provide a precedent for a molecular genetic taxonomy of the genus Symbiodinium.
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Selected References
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- Blank R. J., Trench R. K. Speciation and symbiotic dinoflagellates. Science. 1985 Aug 16;229(4714):656–658. doi: 10.1126/science.229.4714.656. [DOI] [PubMed] [Google Scholar]
- Britten R. J. Rates of DNA sequence evolution differ between taxonomic groups. Science. 1986 Mar 21;231(4744):1393–1398. doi: 10.1126/science.3082006. [DOI] [PubMed] [Google Scholar]
- Herzog M., Maroteaux L. Dinoflagellate 17S rRNA sequence inferred from the gene sequence: Evolutionary implications. Proc Natl Acad Sci U S A. 1986 Nov;83(22):8644–8648. doi: 10.1073/pnas.83.22.8644. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lenaers G., Scholin C., Bhaud Y., Saint-Hilaire D., Herzog M. A molecular phylogeny of dinoflagellate protists (pyrrhophyta) inferred from the sequence of 24S rRNA divergent domains D1 and D8. J Mol Evol. 1991 Jan;32(1):53–63. doi: 10.1007/BF02099929. [DOI] [PubMed] [Google Scholar]
- Medlin L., Elwood H. J., Stickel S., Sogin M. L. The characterization of enzymatically amplified eukaryotic 16S-like rRNA-coding regions. Gene. 1988 Nov 30;71(2):491–499. doi: 10.1016/0378-1119(88)90066-2. [DOI] [PubMed] [Google Scholar]
- Olsen G. J., Lane D. J., Giovannoni S. J., Pace N. R., Stahl D. A. Microbial ecology and evolution: a ribosomal RNA approach. Annu Rev Microbiol. 1986;40:337–365. doi: 10.1146/annurev.mi.40.100186.002005. [DOI] [PubMed] [Google Scholar]
- Rowan R., Powers D. A. A molecular genetic classification of zooxanthellae and the evolution of animal-algal symbioses. Science. 1991 Mar 15;251(4999):1348–1351. doi: 10.1126/science.251.4999.1348. [DOI] [PubMed] [Google Scholar]
- Saiki R. K., Gelfand D. H., Stoffel S., Scharf S. J., Higuchi R., Horn G. T., Mullis K. B., Erlich H. A. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science. 1988 Jan 29;239(4839):487–491. doi: 10.1126/science.2448875. [DOI] [PubMed] [Google Scholar]
- Sogin M. L., Elwood H. J., Gunderson J. H. Evolutionary diversity of eukaryotic small-subunit rRNA genes. Proc Natl Acad Sci U S A. 1986 Mar;83(5):1383–1387. doi: 10.1073/pnas.83.5.1383. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sogin M. L., Gunderson J. H. Structural diversity of eukaryotic small subunit ribosomal RNAs. Evolutionary implications. Ann N Y Acad Sci. 1987;503:125–139. doi: 10.1111/j.1749-6632.1987.tb40603.x. [DOI] [PubMed] [Google Scholar]
- Taylor F. J. On dinoflagellate evolution. Biosystems. 1980;13(1-2):65–108. doi: 10.1016/0303-2647(80)90006-4. [DOI] [PubMed] [Google Scholar]
- Yanisch-Perron C., Vieira J., Messing J. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene. 1985;33(1):103–119. doi: 10.1016/0378-1119(85)90120-9. [DOI] [PubMed] [Google Scholar]