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. 2016 May 24;6(8):1205–1219. doi: 10.7150/thno.15083

Figure 5.

Figure 5

Axl mediates Dox resistance in MCF-7/ADR cells through Akt/GSK3β/β-catenin/ZEB1 cascade. (A) MCF-7/ADR cells transfected by si-Akt, si-β-catenin or si-control were cultured in the absence or presence of 10 µg/ml Dox for 48 h. Relative cell survival was analyzed by the CCK8 assay. (B-C) Ectopic expression of β-catenin significantly rescued cell survival in Axl-depleted cells. (D-E) Both mRNA and protein levels were detected in MCF-7/ADR cells transfected with si-Axl or si-β-catenin, respectively. (F) ChIP assay was performed with antibody against β-catenin or control IgG in MCF-7/ADR cells. The immunoprecipitated DNA was analyzed by PCR followed by agarose gel electrophoresis. (G) Western blot analysis of ZEB1 protein level in MCF-7/ADR cells transfected with ZEB1 si-RNAs or a control si-RNA. (H) MCF-7/ADR cells transfected with si-ZEB1 or si-control were cultured in the absence or presence of 10 µg/ml Dox for 48 h. Relative cell survival was analyzed by CCK8 assay. (I-J) MCF-7/ADR cells transfected by si-ZEB1 or si-control were cultured in the presence of 10 µg/ml Dox for 48 h. Western blot analysis and immunofluorescent staining for γH2AX were performed. *P<0.05, **P<0.01.