FIG 2 .
The abundance of TBP2 is increased by inhibition of 20S proteasomes. (A) Abundance of TBP2 in wild-type (wt) cells treated with an inhibitor of proteasomal CPs (bortezomib, +) or a mock control (dimethylformamide [DMF], −). (B) Right, TBP2 abundance in GZ138 (PtnaA-psmB) cells grown in medium containing or lacking tryptophan, as well as wt cells grown in medium lacking tryptophan, for the indicated times. Quantification of relative TBP2 protein levels is expressed as fold change from time zero, which was set at 1.0. Left, TBP2-StrepII was coexpressed with Flag-SAMP2 and monitored by anti-StrepII antibody IB. Equal loading was confirmed by CB staining. Experiments were performed in at least biological duplicates, and representative images are shown. Data were quantified by ImageJ. See Materials and Methods for details.