Table 2.
Total number of reads | 27962127 × 2 (paired) |
Number of reads mapped to contigs | 19233474 × 2 + 7645742 (single) |
Number of multi-edge-spanning reads | 1893157 |
Number of resolveda single-edge transcripts (length) | 112527 (32216351 bp) |
Number of partial a single-edge transcripts (length) | 2573 (340276 bp) |
Total number of single-edgeb transcripts (length) | 115100 (32556627 bp) |
Number of resolved of multi-edge transcripts (length) | 20903 (4596622 bp) |
Number of partial multi-edge transcripts (length) | 552 (110063 bp) |
Total number of multi-edgeb (length) transcripts (length) | 21455 (4706685 bp) |
Total number of transcripts (length) | 177463 (40456052 bp) |
Proportion of multi-edge transcripts (in length) | 15.7% (11.6%) |
Only transcripts of at least 100 bp were considered in this summary.
aPartial transcripts: the transcripts that are not fully resolved by TAG (i.e. the edge sequences); Resolved transcripts: the transcripts that are resolved by TAG and therefore likely represent full-length transcripts.
bSingle-edge transcripts: the transcripts reported by TAG that are fully contained within edges (contig) in the de Bruijn graph of the metagenome assembly (they can be considered as the results of a baseline reference-based metatranscriptome assembly approach that uses the contigs as the reference); Multi-edge transcripts: the transcripts reported by TAG that span multiple edges in the de Bruijn graph.