A) Slightly oblique section through the cortex of an internodal cell. Cell wall (cw), charasomes (asterisks), endoplasmic reticulum (arrow), vesicles (v) and mitochondria (m) are not labelled. Unspecific accumulation of gold particles is seen in the chloroplast (c). B–G) Longitudinal sections through the endoplasm. Numerous gold particles (arrow heads) are seen at multivesicular endosomes (B–D), but not at a Golgi body (E). Gold particles are absent from multivesicular endosomes treated with pre-immune serum instead of primary antibody (F, G; negative controls). Bars are 500 nm.