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. Author manuscript; available in PMC: 2016 Jun 8.

Published in final edited form as: Stroke. 2011 Nov 10;43(2):524–531. doi: 10.1161/STROKEAHA.111.635672

Association of cell death markers with PI+ cells after intracerebral hemorrhage. (A) Colocalization of caspase substrates and TUNEL with permeable cells 48 h after ICH. (a) In vivo PI stains permeable cells red. (b) DEVD labeling of the brain section in (a). (c) Overlay of a and b. (d) Overlay of a brain section labeled with in vivo PI (red) and ex vivo TUNEL (green). Only a few cells stained green and not red (PI−/TUNEL+). Scale bar, 40 um for each panel. (B) Quantitative analyses of PI+ cells labeled ex vivo with DEVD (e) or TUNEL (f) at 48 h after ICH. Values shown are averages of peripheral and core regions. *p < 0.05 vs. PI+/DEVD− and PI+/DEVD+ and PI+/TUNEL− and PI+/TUNEL+.

Association of cell death markers with PI+ cells after intracerebral hemorrhage. (A) Colocalization of caspase substrates and TUNEL with permeable cells 48 h after ICH. (a) In vivo PI stains permeable cells red. (b) DEVD labeling of the brain section in (a). (c) Overlay of a and b. (d) Overlay of a brain section labeled with in vivo PI (red) and ex vivo TUNEL (green). Only a few cells stained green and not red (PI−/TUNEL+). Scale bar, 40 um for each panel. (B) Quantitative analyses of PI+ cells labeled ex vivo with DEVD (e) or TUNEL (f) at 48 h after ICH. Values shown are averages of peripheral and core regions. *p < 0.05 vs. PI+/DEVD− and PI+/DEVD+ and PI+/TUNEL− and PI+/TUNEL+.