. Author manuscript; available in PMC: 2017 Apr 1.

Published in final edited form as: Differentiation. 2016 Feb 5;91(4-5):152–158. doi: 10.1016/j.diff.2016.01.007

Table 1.

Serum-free DMEM:F12 basal medium for propagation of metanephric mesenchymal progenitors. All stock solutions are aliquoted to avoid repeated freeze-thaw cycles and stored at −20°C.

Component	Stock Concentration	Medium Concentration
glutamine	200 mM	2.0 mM
gentamicin	50 mg/ml	50 μg/ml
transferrin, holo, human plasma	5 mg/ml in PBS	5 μg/ml
insulin, bovine	5 mg/ml in 0.006 N HCl	5 μg/ml
sodium selenite	1 × 10⁻⁵ M in PBS	1 × 10⁻⁸ M
hydrocortisone	1 × 10⁻⁴ M in 95% EtOH	1 × 10⁻⁷ M
triiodothyronine	1 × 10⁻⁶ M in DMSO	1 × 10⁻⁹ M