Abstract
Neochromosomes are a little-studied class of chromosome-scale mutations that drive some cancers. By sequencing isolated neochromosomes from liposarcomas, we recently defined their structure at single-nucleotide resolution and proposed a model for their life history. Here, we summarize that work, highlighting significant aspects and providing historical context and insight into the discovery process.
Keywords: neochromosome, chromothripsis, breakage-fusionbridge mechanism, liposarcoma, cancer evolution, computational modelling
Neochromosomes are giant extra chromosomes that are found in 3% of cancers overall1 but are particularly common in certain cancer subtypes, including well-differentiated and de-differentiated liposarcomas (WD/DD-LPS). Neochromosomes may be circular or linear, possess centromeres and telomeres (in the linear form), and can harbor oncogenes at high copy number that drive tumor development. They were probably first observed in the 1950s by the Swedish geneticist Albert Levan as ring chromosomes in tumors,2 but were not identified as a distinct cancer-causing entity until the 1990s.3 How neochromosomes form has remained a mystery.
Low-resolution analyses of WD/DD-LPS neochromosomes using fluorescent in situ hybridization (FISH) and copy number arrays provided an approximate idea of DNA content, and an (under-) estimate of their size and copy number. This led to the hypothesis that the breakage-fusion-bridge (BFB) mechanism4 might be involved in their formation,1,3 but no solid proof of this existed. BFB was discovered by Barbara McClintock in maize in 1951. It is initiated by telomere loss: single telomere loss leads to the formation of a dicentric linear chromosome during replication, whereas loss of both telomeres leads to the formation of a ring chromosome. During replication, DNA crossover events between the sister chromatids can occur. An odd number of crossovers leads to an interlocked and dicentric ring. Dicentric chromosomes form anaphase bridges and are torn apart during cell division. This leads to unequal inheritance of DNA by daughter cells and possible selection of some cell subpopulations.1
In our recent publication,5 we describe the analysis of sequencing data from purified neochromosomes obtained from WD/DD-LPS cell lines by flow cytometric cell sorting and whole-genome sequence data from patient tumors. Flow sorting of chromosomes works by selecting DNA molecules based on the intensity of 2 fluorescent dyes that are markers of size and GC-content. We purified neochromosomal DNA in order to sequence the neochromosome at high coverage. Unavoidably, the rest of the genome was sequenced at low coverage. This can be seen in plots of the neochromosome copy number, which highlight multiple fascinating features in the background of the normal chromosomes (e.g., Fig. 1).
Analysis and interpretation of the neochromosome sequencing data required a high level of creativity. Almost every aspect of analysis (see Fig. 1A) required a specialized method because of the unusual nature of the complex data generated. This included the copy number analysis, which integrated information from spectral karyotyping to calibrate the copy number on the neochromosome, fusion detection, classification of neochromosomal material, and finally a mathematical model of the mutational mechanisms that shaped the neochromosome over time. In a similar vein, we repeatedly found that we had to invent new terms to describe and discuss the data—including the term ‘neochromosome’ itself.
The sequencing data provided the first high-resolution view of a neochromosome, allowing us to describe their architecture in detail (Fig. 1B). Several observations suggested the involvement of chromothripsis, the shattering of a chromosome in a single catastrophic event6 that was first identified in tumors that were otherwise genomically stable. The occurrence of a chromothriptic event is not observed directly, but rather inferred from the ruins of the chromosomes that remain. A set of statistical criteria have been proposed to test for chromothripsis,7 but they are strongly motivated by the ideal case of a largely quiescent genome and must be adapted for more complex cases (see the Supplementary Information of references 5 and 8 for a detailed discussion). Our data were the antithesis of this: nearly every chromosome was involved and showed extreme copy number amplification. Nevertheless, the telomeric regions of the neochromosome showed the classic signature of chromothripsis and multiple other lines of evidence supported the notion that chromothripsis underlies the highly amplified regions of chromosome 12 present in all neochromosomes. In particular, we discovered that the fundamental building blocks (we used the term contiguous genomic regions or CGRs; Fig. 1B) were rearranged and fused edge-to-edge in an intrachromosomal walk, suggestive of an early chromothriptic event involving chromosome 12 only.
The key to demonstrating that both chromothripsis and BFB were involved in the formation of the neochromosome was the development of a mathematical model of these mechanisms. There were really 4 components to our model: a stochastic simulation of circular BFB, a simple model of selection, a model of chromothripsis, and a way to compare the model with the data. Because of the lack of detailed information about these mechanisms, our approach was to try to make few assumptions, keep the models simple, and use empirical data where possible. How we compared the model results to the data was the most critical part. We ran the model hundreds of times and compared summary statistics of the predictions from each run with summary statistics of the data. We found that we could not fit the BFB-only model to the data for any parameter choices, whereas the BFB plus chromothripsis model easily fitted the data.
Through detailed analysis of sequencing data from liposarcoma neochromosomes, development of specialized methods for genotyping, hypothesis testing using computational modeling, and integration with observations of centromeres across multiple neochromosomes, we were able to infer the life history of these oncogenic chromosomal-scale mutations.
Disclosure of Potential Conflicts of Interest
No potential conflicts of interest were disclosed.
Funding
DMT and ATP were supported by a National Health and Medical Research Council (NHMRC) Project Grant (1004022) and the Liddy Shriver Sarcoma Initiative. DMT was supported by an NHMRC Senior Research Fellowship (1003929). ATP was supported by an NHMRC Career Development Fellowship (1003856) and an NHMRC Program Grant (1054618). The work benefitted from support by the Victorian State Government Operational Infrastructure Support and Australian Government NHMRC IRIISS.
References
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