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. 2016 Feb 8;7(10):11349–11363. doi: 10.18632/oncotarget.7239

Figure 1. Expression ofHNF4α in clinical gastric tissues and its role in regulating gastric cell proliferation.

Figure 1

A. IHC staining of HNF4α in atrophic gastritis and gastric cancer samples. Representative images are shown here (magnification 100x,400x; Scale bars:200μm,50μm). B. The mRNA levels of HNF4α in 30 atrophic gastritis samples and 30 gastric cancers were measured by real-time PCR. The horizontal bars indicate the mean value of each sample group. Mann-Whitney U-test was used to calculate the P value. C. HNF4α mRNA expression levels in indicated gastric cancer cell lines and immortalized gastric cells GES-1. ***p<0.001 by Student's t-test. D. and E. HNF4α mRNA and protein levels in indicated cells transfected with HNF4α siRNA or HNF4α over-expression plasmid. *p<0.05, **p<0.01, ***p<0.001 by Student's t-test. F. Foci formation after cells transfected HNF4α siRNA or HNF4α over-expression plasmid. *p<0.05, **p<0.01 by Student's t-test. Representative images are shown here from three independent biological replicates. G. Western blot shows loss of HNF4α in BGC-823 cells transfected with lenti-HNF4α shRNA; Tumors formed in nude mice induced by BGC-823 cells transfected with lenti-negative control group(NC)and lenti-HNF4α shRNA group(Hsh). H.Tumor growth curve and tumor weight of NC group and Hsh group. *p<0.05 by Student's t-test.