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. 2016 Feb 13;7(10):11733–11743. doi: 10.18632/oncotarget.7367

Figure 5. Knockdown of SRPK1 in MALAT1-activated SW480 cells (RNAa-MALAT1) attenuated AKAP-9 expression by attenuating the level of SRSF1 phosphorylation.

Figure 5

(A) Knockdown of SRPK1 in RNAa-MALAT1 SW480 cells attenuated phospholated SRSF1 and AKAP-9 protein expression. (B) The phospholated SRSF1 and AKAP-9 protein levels were normalized α-Tubulin. *P < 0.01 compared to the control group (Scramle-MALAT1), **P < 0.01 compared to the control group (RNAa-MALAT1/siCtrl), n = 3. (C) The phospholated SRSF1 protein in nucleus and cytoplasm were detected by Western blot analysis using mAb1H4 antibody respectively. The phospholated SRSF1 protein is enriched in nucleus. (D) The phospholated SRSF1 protein levels were normalized to Histone. *P < 0.05 compared to the control group (Scramle-MALAT1), **P < 0.01 compared to the control group (RNAa-MALAT1/siCtrl), n = 3.