Figure 7.

Genomes of the evolved lineages with NIb moved to an alternative position. Mutations were detected using NGS data of the evolved TEV-NIb₁-ΔNIb₉ and TEV-NIb₂-ΔNIb₉ lineages compared to their ancestral lineages. The wild-type TEV is given for comparative purposes. The names on the left identify lineages (e.g., 9WL1 is the final population of 9-week passages, lineage 1). Filled circles and open circles represent nonsynonymous and synonymous substitutions, respectively. Black substitutions occur in only one lineage, whereas color-coded substitutions are repeated in two or more lineages. Note that the mutations are present at different frequencies, as reported by SAMtools (>10%).