Fig 4A shows expression of CD-9 in small, medium, and large tumor bearing mice. There are erroneously 5 lanes in the figure; the fifth lane was a duplication of large tumor sample from another mouse. Please view the correct Fig 4, without the extraneous lane in panel A, here.
Fig 4. Exosome isolation from plasma is validated by the presence of exosome markers.
a) CD9 was present in exosomes derived from LNCaP xenograft mice bearing small, medium and large LNCaP tumours whereas the control mouse serum lacked CD9. GRP94, a known endoplasmic reticulum protein which is used as a negative control was absent in the exosomes suggesting enrichment b) LAMP2 was present in exosomes derived from PCa patient plasma whereas absence of LAMP2 in whole plasma indicated successful enrichment. c) Alix was present in exosomes derived from patient plasma at different exosomal protein concentrations.
Supporting Information
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Reference
- 1.Kharmate G, Hosseini-Beheshti E, Caradec J, Chin MY, Tomlinson Guns ES (2016) Epidermal Growth Factor Receptor in Prostate Cancer Derived Exosomes. PLoS ONE 11(5): e0154967 doi: 10.1371/journal.pone.0154967 [DOI] [PMC free article] [PubMed] [Google Scholar]
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