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. 1992 May 15;89(10):4358–4362. doi: 10.1073/pnas.89.10.4358

Immunostimulatory human urinary protein.

E Fontan 1, H Saklani-Jusforgues 1, R M Fauve 1
PMCID: PMC49081  PMID: 1584770

Abstract

We have previously extracted a protein from inflammatory mouse granuloma that fully protects normal mice against lethal Listeria monocytogenes infection. We now report that polyclonal antibodies directed against this protein react with a human urinary fraction that also provides full protection of normal mice against L. monocytogenes. Murine monoclonal antibodies completely inhibit the protective activity of the human urinary fraction. We have purified to apparent homogeneity a human glycoprotein of 43 kDa (HGP.43), pI = 3.2 +/- 0.2. HGP.43 injected i.v. at 250 micrograms/kg fully protected mice against a lethal inoculum of L. monocytogenes. Such resistance followed injection of HGP.43, 4 days before and, still significantly, 8 hr after Listeria infection. In scid mice lacking T and B cells, similar resistance to L. monocytogenes was observed. Inflammatory murine macrophages became cytostatic against Lewis carcinoma cells after incubation with 1.5 nM HGP-43.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Czuprynski C. J., Brown J. F. Recombinant murine interleukin-1 alpha enhancement of nonspecific antibacterial resistance. Infect Immun. 1987 Sep;55(9):2061–2065. doi: 10.1128/iai.55.9.2061-2065.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Eckert K., Granetzny A., Fischer J., Nexø E., Grosse R. A Mr 43,000 epidermal growth factor-related protein purified from the urine of breast cancer patients. Cancer Res. 1990 Feb 1;50(3):642–647. [PubMed] [Google Scholar]
  3. Fauve R. M., Jusforgues H., Hevin B. Maintenance of granuloma macrophages in serum-free medium. J Immunol Methods. 1983 Nov 25;64(3):345–351. doi: 10.1016/0022-1759(83)90442-8. [DOI] [PubMed] [Google Scholar]
  4. Fontan E., Fauve R. M., Hevin B., Jusforgues H. Immunostimulatory mouse granuloma protein. Proc Natl Acad Sci U S A. 1983 Oct;80(20):6395–6398. doi: 10.1073/pnas.80.20.6395. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Fontan E., Fauve R. M. Increased phagocytic activity in mice treated by a mouse granuloma protein. Ann Inst Pasteur Immunol. 1986 Jul-Aug;137D(1):93–107. [PubMed] [Google Scholar]
  6. Fontan E., Fauve R. M. Inflammation and anti-tumor resistance. IV. Induction of cytostatic activity of murine peritoneal cells by a mouse granuloma protein. Int J Cancer. 1988 Aug 15;42(2):267–272. doi: 10.1002/ijc.2910420221. [DOI] [PubMed] [Google Scholar]
  7. Gregory H. Isolation and structure of urogastrone and its relationship to epidermal growth factor. Nature. 1975 Sep 25;257(5524):325–327. doi: 10.1038/257325a0. [DOI] [PubMed] [Google Scholar]
  8. Havell E. A. Evidence that tumor necrosis factor has an important role in antibacterial resistance. J Immunol. 1989 Nov 1;143(9):2894–2899. [PubMed] [Google Scholar]
  9. Kiderlen A. F., Kaufmann S. H., Lohmann-Matthes M. L. Protection of mice against the intracellular bacterium Listeria monocytogenes by recombinant immune interferon. Eur J Immunol. 1984 Oct;14(10):964–967. doi: 10.1002/eji.1830141019. [DOI] [PubMed] [Google Scholar]
  10. Lu L., Shen R. N., Lin Z. H., Aukerman S. L., Ralph P., Broxmeyer H. E. Anti-tumor effects of recombinant human macrophage colony-stimulating factor, alone or in combination with local irradiation, in mice inoculated with Lewis lung carcinoma cells. Int J Cancer. 1991 Jan 2;47(1):143–147. doi: 10.1002/ijc.2910470125. [DOI] [PubMed] [Google Scholar]
  11. MACKANESS G. B. Cellular resistance to infection. J Exp Med. 1962 Sep 1;116:381–406. doi: 10.1084/jem.116.3.381. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Mayer P., Schütze E., Lam C., Kricek F., Liehl E. Recombinant murine granulocyte-macrophage colony-stimulating factor augments neutrophil recovery and enhances resistance to infections in myelosuppressed mice. J Infect Dis. 1991 Mar;163(3):584–590. doi: 10.1093/infdis/163.3.584. [DOI] [PubMed] [Google Scholar]
  13. Mount C. D., Lukas T. J., Orth D. N. Characterization of a high-molecular-weight form of epidermal growth factor in an extract of human urine. Arch Biochem Biophys. 1987 May 15;255(1):1–7. doi: 10.1016/0003-9861(87)90287-6. [DOI] [PubMed] [Google Scholar]
  14. Nakane A., Minagawa T., Kato K. Endogenous tumor necrosis factor (cachectin) is essential to host resistance against Listeria monocytogenes infection. Infect Immun. 1988 Oct;56(10):2563–2569. doi: 10.1128/iai.56.10.2563-2569.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. North R. J. Cellular mediators of anti-Listeria immunity as an enlarged population of short lived, replicating T cells. Kinetics of their production. J Exp Med. 1973 Aug 1;138(2):342–355. doi: 10.1084/jem.138.2.342. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. PIERCE C. H., DUBOS R. J., SCHAEFER W. B. Multiplication and survival of tubercle bacilli in the organs of mice. J Exp Med. 1953 Feb 1;97(2):189–206. doi: 10.1084/jem.97.2.189. [DOI] [PMC free article] [PubMed] [Google Scholar]

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