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. 2016 Mar 19;7:62–72. doi: 10.1016/j.ebiom.2016.03.023

Fig. 6.

Fig. 6

The effect of lincRNAs downregulation on cell proliferation and migration.

(a) PCAN-2 and PCAN-3 lincRNAs can be efficiently knocked down in MDA-MB-231 and MCF-7 cell lines. Bars represent RT-qPCR results of PCAN-2 and PCAN-3 expression. siRNA lincRNA bars show mean expression (n = 3) with S.D. normalized to the control condition. (b) Transient knockdown of PCAN-2 and PCAN-3 inhibits the growth rate of MDA-MB-231 cells. 30 h after transfection (time point “0”) 400 cells were seeded in 96-well plates and processed for luminescent cell viability assay at indicated time points. Data points represent mean value (n = 3), error bars, S.D. *, P < 0.05, **P < 0.01. (c) lincRNA knockdown inhibits migration of MDA-MB-231 and MCF-7 cells in wound-healing assay. Cells were transiently transfected 30 h before making scratches in the cell monolayer. Cell migration rate was analysed with time-lapse microscopy. Red lines – cells tracks analysed over 24 h. Size bars – 100 μm. (d) Quantification of MDA-MB-231 and MCF-7 cells migration distance over 24-h time period. Bars – value of mean migration distance, error bars – S.D. (n = 20–25 analysed cells), ***P < 0.001.