Abstract
The antibody and cell mediated immune responses were investigated in inbred Lewis rats with experimental allergic neuritis (EAN) induced by either P2, a protein purified from the bovine cauda equina nerve roots, or whole bovine nerve root myelin. In the P2 immunised animals both antibodies to P2 detected by radioimmunoassay and cell-mediated immunity to P2 assayed by skin testing appeared before the onset of EAN and persisted during and after the disease. In the myelin immunised animals the antibody titres were lower and somewhat delayed and the skin tests became negative at the height of the disease. Complement-fixing antibodies to galactocerebroside, which have been implicated in the production of demyelination under some circumstances, could not be detected in the serum after immunisation with either P2 or myelin. EAN was transferred passively with lymph node cells from rats immunised with either P2 or myelin although anti-P2 antibodies could not be detected in the serum of recipients with EAN. The results favour a cell-mediated immune response to P2 as the most important pathogenetic mechanism in EAN induced wtih whole myelin in the rat.
Full text
PDF




Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- ASTROM K. E., WAKSMAN B. H. The passive transfer of experimental allergic encephalomyelitis and neuritis with living lymphoid cells. J Pathol Bacteriol. 1962 Jan;83:89–106. doi: 10.1002/path.1700830112. [DOI] [PubMed] [Google Scholar]
- Abramsky T. O., Teitelbaum D., Arnon R. Experimental allergic neuritis induced by a basic neuritogenic protein (P1L) of human peripheral nerve origin. Eur J Immunol. 1977 Apr;7(4):213–217. doi: 10.1002/eji.1830070405. [DOI] [PubMed] [Google Scholar]
- Arnason B. G., Chelmicka-Szorc E. Passive transfer of experimental allergic neuritis in Lewis rats by direct injection of sensitized lymphocytes into sciatic nerve. Acta Neuropathol. 1972;22(1):1–6. doi: 10.1007/BF00687545. [DOI] [PubMed] [Google Scholar]
- Hahn A. F., Gilbert J. J., Feasby T. E. Passive transfer of demyelination by experimental allergic neuritis serum. Acta Neuropathol. 1980;49(3):169–176. doi: 10.1007/BF00707103. [DOI] [PubMed] [Google Scholar]
- Hoffman P. M., Powers J. M., Weise M. J., Brostoff S. W. Experimental allergic neuritis. I. Rat strain differences in the response to bovine myelin antigens. Brain Res. 1980 Aug 18;195(2):355–362. doi: 10.1016/0006-8993(80)90071-2. [DOI] [PubMed] [Google Scholar]
- Hughes R. A., Gray I. A., Clifford-Jones R., Stern M. A. Lymphocyte transformation in the presence of myelin basic protein in multiple sclerosis and control subjects. Acta Neurol Scand. 1979 Aug;60(2):65–76. doi: 10.1111/j.1600-0404.1979.tb02953.x. [DOI] [PubMed] [Google Scholar]
- Hughes R. A., Stedronska J. The susceptibility of rat strains to experimental allergic encephalomyelitis. Immunology. 1973 May;24(5):879–884. [PMC free article] [PubMed] [Google Scholar]
- Kadlubowski M., Hughes R. A., Gregson N. A. Experimental allergic neuritis in the Lewis rat: characterization of the activity of peripheral myelin and its major basic protein,P2. Brain Res. 1980 Feb 24;184(2):439–454. doi: 10.1016/0006-8993(80)90811-2. [DOI] [PubMed] [Google Scholar]
- Kadlubowski M., Hughes R. A. Identification of the neuritogen for experimental allergic neuritis. Nature. 1979 Jan 11;277(5692):140–141. doi: 10.1038/277140a0. [DOI] [PubMed] [Google Scholar]
- Kadlubowski M., Hughes R. A. The neuritogenicity and encephalitogenicity of P2 in the rat, guinea-pig and rabbit. J Neurol Sci. 1980 Nov;48(2):171–178. doi: 10.1016/0022-510x(80)90198-7. [DOI] [PubMed] [Google Scholar]
- Lampert P. W. Mechanism of demyelination in experimental allergic neuritis. Electron microscopic studies. Lab Invest. 1969 Feb;20(2):127–138. [PubMed] [Google Scholar]
- Nyland H., Aarli J. A. Guillain-Barré syndrome: demonstration of antibodies to peripheral nerve tissue. Acta Neurol Scand. 1978 Jul;58(1):35–43. doi: 10.1111/j.1600-0404.1978.tb02857.x. [DOI] [PubMed] [Google Scholar]
- Ortiz-Ortiz L., Weigle W. O. Cellular events in the induction of experimental allergic encephalomyelitis in rats. J Exp Med. 1976 Sep 1;144(3):604–616. doi: 10.1084/jem.144.3.604. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SHAW C. M., ALVORD E. C., Jr, KAKU J., KIES M. W. CORRELATION OF EXPERIMENTAL ALLERGIC ENCEPHALOMYELITIS WITH DELAYED-TYPE SKIN SENSITIVITY TO SPECIFIC HOMOLOGOUS ENCEPHALITOGEN. Ann N Y Acad Sci. 1965 Mar 31;122:318–331. doi: 10.1111/j.1749-6632.1965.tb20216.x. [DOI] [PubMed] [Google Scholar]
- Saida T., Saida K., Dorfman S. H., Silberberg D. H., Sumner A. J., Manning M. C., Lisak R. P., Brown M. J. Experimental allergic neuritis induced by sensitization with galactocerebroside. Science. 1979 Jun 8;204(4397):1103–1106. doi: 10.1126/science.451555. [DOI] [PubMed] [Google Scholar]
- Sarvas H. O., Milek D. J., Weise M. J., Carnow T. B., Fudenberg H. H., Brostoff S. W. Radioimmunoassay for the P2 protein of bovine peripheral nerve myelin. J Immunol. 1980 Feb;124(2):557–564. [PubMed] [Google Scholar]
- Sheremata W., Colby S., Karkhanis Y., Eylar E. H. Cellular hypersensitivity to basic myelin (P2) protein in the Guillain-Barré syndrome. Can J Neurol Sci. 1975 May;2(2):87–90. doi: 10.1017/s0317167100020059. [DOI] [PubMed] [Google Scholar]
- Smith M. E., Forno L. S., Hofmann W. W. Experimental allergic neuritis in the Lewis rat. J Neuropathol Exp Neurol. 1979 Jul;38(4):377–391. doi: 10.1097/00005072-197907000-00003. [DOI] [PubMed] [Google Scholar]
- Suzuki M., Kitamura K., Uyemura K., Ogawa Y., Ishihara Y., Matsuyama H. Neuritogenic activity of peripheral nerve myelin proteins in Lewis rats. Neurosci Lett. 1980 Oct 2;19(3):353–358. doi: 10.1016/0304-3940(80)90287-6. [DOI] [PubMed] [Google Scholar]