Figure 1.

Mouse cancer cells with highly metastatic and aggressive phenotype express Cripto-1. (A) tumor cell lysates from lines on B6 background, RetV, MCA205, B16F10 and B16F1 were checked by protein gel blot for mouse Cripto-1 expression and compared relatively to β-actin. The D2F2 mouse Cripto-1 transfectant (D2F2mCR) was included as positive control. (B) B16F10 tumor cells were grown on low attachment plates for one to three passages (P1, P2 and P3) to enrich for tumor initiating cells and cell lysates were generated from each passage. (C) Expression levels of mouse cripto-1 were assessed from healthy mouse tissue from the GSE1133 data set and represented sorted with highest expression to the left.