(A) Palmitoleic acid-evoked calcium responses at the Or47b axon terminals in 7-day old males treated with solvent (ethanol) or precocene. (B) Dose-response curves of Or47b ORN in response to palmitoleic acid. Precocene significantly decreased the response magnitude of Or47b ORNs, mean± s.e.m. (n=8–9). (C) Copulation percentage of precocene-treated 7-day old males. (D–F) Similar to (A–C), with 2-day old males treated with methoprene. (D–E) Methoprene markedly enhanced the response magnitude of the Or47b ORNs to palmitoleic acid, compared to control, mean± s.e.m. (n=8–9). (F) Methoprene conferred a copulation advantage on WT but not Or47b2 mutant males. For imaging experiments, statistical significance was determined by t-test (*, p<0.05; **, p<0.01). For behavioral experiments, Chi-square test was used to determine significance above chance (dashed lines in C and F). Results are from five parallel experiments, 20–22 matches per experiment.