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. 1992 May 15;89(10):4514–4518. doi: 10.1073/pnas.89.10.4514

Methods for the large-scale synthesis of psoralen furan-side monoadducts and diadducts.

H P Spielmann 1, S S Sastry 1, J E Hearst 1
PMCID: PMC49113  PMID: 1584785

Abstract

We report methods for the preparation of multimicromole quantities of extremely pure uniquely photo-adducted psoralen-DNA furan-side monoadducts and diadducts (cross-links). The methods use high-intensity krypton and argon ion lasers in the photoreactions and HPLC methods to purify the required oligonucleotides containing the photoadducts. With these methods we have synthesized 2-3 mumol of 8-mer psoralen furan-side monoadduct and diadduct. These methods allow one to generate large amounts of psoralenated DNA oligonucleotides and facilitate their study by NMR and x-ray crystallography.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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