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. 2016 Jan 18;7(11):12761–12782. doi: 10.18632/oncotarget.6938

Figure 6. HMGA2 silencing reduces telomeric DNA bound to TRF2.

Figure 6

Telomere-Chromatin immunoprecipitation was performed with TRF2 antibody in the presence of HMGA2 (−dox) and at HMGA2low conditions (+dox). The presence of telomeric DNA was quantified by real time quantitative PCR in TRF2 immunoprecipitated and purified DNA [73]. Enrichment of telomeric DNA in telomere-ChIP upon TRF2 IP was normalized to the corresponding IgG control. A. A significant reduction in fold enrichment of telomeric DNA was observed upon knockdown of HMGA2 (+dox) p<0.001***. B. TRF2 detection, in the corresponding ChIP samples, by Western blot following TRF2 pull-down is shown compared to IgG control. Quantitative data are shown as mean +/− SEM; ***p<0.001.