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. 2015 Dec 8;6(6):479–489. doi: 10.1080/19491034.2015.1128608

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© 2015 The Author(s). Published with license by Taylor & Francis Group, LLC

This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License http://creativecommons.org/licenses/by-nc/3.0/, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

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Figure 2. — Nesprin-2 KD cells have a hypoactive DDR. (A) Immunofluorescence analysis after UV treatment of control and Nesprin-2 KD HaCaT cells with antibodies against Nesprin-2 (pAbK1), γ-H2AX and DAPI. Scale bar, 10 µm. (B) Graphical representation of the average number of γ-H2AX foci per nucleus. Foci ≥ 0.7 µm were counted, analysis was with cellprofiler image analysis software (mean ± s.d, p value, 0.0084). 100 cells were analyzed.