Figure 3.

let-7 regulates nucleolar size in vulva cells by controlling fibrillarin expression. (A) DIC microscopy of the vulva cells of let-7(n2853) and let-7(n2853); ncl-1(e1942) worms, at the permissive (15°C) or non-permissive (25°C) temperatures. Insets represent enlarged images of the boxed regions in the corresponding figures. Arrowheads point to the nucleoli of the vulva cells. Scale bar, 10 μm. (B) Quantitative representation of the results shown in (A), illustrating the nucleolar sizes in the vulva. Asterisks signify the difference in sizes observed at the indicated temperatures, where ***P < 0.001, n = 22–36 for let-7(n2853). NS = not significant, n = 100–110 for ncl-1(e1942); let-7(n2853). (C) Western blot analysis of FIB-1 and an Actin control in the indicated strains. Numbers below represent the relative levels of FIB-1 protein expression (normalized to the control sample of each pair-wise comparison). Adapted from Yi et al., (2015)²³ with permission from PLoS Genetics.