HSC were treated with lentiviral vectors harboring distinct shRNA sequences for Yap1(YAP1D1, YAP1D2) or non-targeting control vectors (NT) and harvested for western blot and qRT-PCR analysis. Top: western blot showing Yap1 and Lamin B (nuclear protein loading control). Bottom: composite panel showing Desmin, αSMA, Snail, Collagen 1a1, Gli1 mRNA. Results were expressed as fold change relative to NT treated HSC; mean ± SEM were graphed; *p<0.05 vs. NT HSC.