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. Author manuscript; available in PMC: 2017 Jul 1.
Published in final edited form as: Hepatology. 2016 Apr 21;64(1):232–244. doi: 10.1002/hep.28542

Figure 5. Hedgehog-responsive MF-HSC regulate Yap1 expression in total liver.

Figure 5

In separate experiments, αSMA-CreERt2/SMOflox/flox double transgenic (DTG) mice were treated with either vehicle (VEH) or TMX, and then sacrificed at 24, 48, 72, and 96h post-PH. Livers were harvested and analysed by immunohistochemistry. (A) Representative Yap1 immunostaining from VEH- and TMX-treated groups at time 0, and 48 h post-PH. (B) Yap1 quantification at time points after PH. Results were expressed as total number of Yap1 (+) cells per HPF; mean ± SEM; white columns represent VEH-treated DTG; black columns represent TMX-treated DTG. Liver RNA was analysed by qRT-PCR. (C) YAP1 mRNA. (D) CTGF mRNA. (E) AREG mRNA. Results were expressed as fold change relative to quiescent (time 0) livers; mean ± SEM were graphed; *p<0.05 vs time-matched VEH-treated DTG.