Skip to main content
PMC home page
Journal of Neurology, Neurosurgery, and Psychiatry logoLink to Journal of Neurology, Neurosurgery, and Psychiatry
. 1975 Sep;38(9):900–910. doi: 10.1136/jnnp.38.9.900

Intercostal muscle biopsy in human neuromuscular disease. Histochemical and electron microscopic studies.

L Z Stern, C M Payne, R Gruener, R M Anderson, L K Hannapel
PMCID: PMC492120  PMID: 127018

Abstract

External intercostal muscle biopsies were examined histochemically and by electron microscopy. The use of this muscle allowed correlation with physiological and pharmacological studies on the same specimens. Changes observed in musclar dystrophy and motor neurone disease resembled those previously described in biopsied limb muscle and underline the particular usefulness of this preparation in the study of human neuromuscular disease.

Full text

PDF
900

Images in this article

901Image
on p.901
902Image
on p.902
903Image
on p.903
904Image
on p.904
905Image
on p.905
906Image
on p.906
907Image
on p.907

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Allen D. E., Johnson A. G., Woolf A. L. The intramuscular nerve endings in dystrophia myotonica--a biopsy study by vital staining and electron microscopy. J Anat. 1969 Jul;105(Pt 1):1–26. [PMC free article] [PubMed] [Google Scholar]
  2. Brooke M. H., Engel W. K. The histographic analysis of human muscle biopsies with regard to fiber types. 1. Adult male and female. Neurology. 1969 Mar;19(3):221–233. doi: 10.1212/wnl.19.3.221. [DOI] [PubMed] [Google Scholar]
  3. Brooke M. H., Kaiser K. K. Muscle fiber types: how many and what kind? Arch Neurol. 1970 Oct;23(4):369–379. doi: 10.1001/archneur.1970.00480280083010. [DOI] [PubMed] [Google Scholar]
  4. CREESE R., DILLON J. B., MARSHALL J., SABAWALA P. B., SCHNEIDER D. J., TAYLOR D. B., ZINN D. E. The effect of neuromuscular blocking agents on isolated human intercostal muscles. J Pharmacol Exp Ther. 1957 Apr;119(4):485–494. [PubMed] [Google Scholar]
  5. ELMQVIST D., HOFMANN W. W., KUGELBERG J., QUASTEL D. M. AN ELECTROPHYSIOLOGICAL INVESTIGATION OF NEUROMUSCULAR TRANSMISSION IN MYASTHENIA GRAVIS. J Physiol. 1964 Nov;174:417–434. doi: 10.1113/jphysiol.1964.sp007495. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. ELMQVIST D., JOHNS T. R., THESLEFF S. A study of some electrophysiological properties of human intercostal muscle. J Physiol. 1960 Dec;154:602–607. doi: 10.1113/jphysiol.1960.sp006599. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. ENGEL W. K., CUNNINGHAM G. G. RAPID EXAMINATION OF MUSCLE TISSUE. AN IMPROVED TRICHROME METHOD FOR FRESH-FROZEN BIOPSY SECTIONS. Neurology. 1963 Nov;13:919–923. doi: 10.1212/wnl.13.11.919. [DOI] [PubMed] [Google Scholar]
  8. Edström L., Nyström B. Histochemical types and sizes of fibres in normal human muscles. A biopsy study. Acta Neurol Scand. 1969;45(3):257–269. doi: 10.1111/j.1600-0404.1969.tb01238.x. [DOI] [PubMed] [Google Scholar]
  9. Elmqvist D., Quastel D. M. A quantitative study of end-plate potentials in isolated human muscle. J Physiol. 1965 Jun;178(3):505–529. doi: 10.1113/jphysiol.1965.sp007639. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. FARBER E., STERNBERG W. H., DUNLAP C. E. Histochemical localization of specific oxidative enzymes. I. Tetrazolium stains for diphosphopyridine nucleotide diaphorase and triphosphopyridine nucleotide diaphorase. J Histochem Cytochem. 1956 May;4(3):254–265. doi: 10.1177/4.3.254. [DOI] [PubMed] [Google Scholar]
  11. Gruener R., Stern L. Z., Payne C., Hannapel L. Hyperthyroid myopathy. Intracellular electrophysiological measurements in biopsied human intercostal muscle. J Neurol Sci. 1975 Mar;24(3):339–349. doi: 10.1016/0022-510x(75)90254-3. [DOI] [PubMed] [Google Scholar]
  12. Hofmann W. W., Birnberger K. L., Harlacher A., Schreiber M. Normal and dystrophic human muscle in vitro: an argument against denervation. Exp Neurol. 1973 Mar-Apr;39(2):249–260. doi: 10.1016/0014-4886(73)90227-6. [DOI] [PubMed] [Google Scholar]
  13. Jennekens F. G., Tomlinson B. E., Walton J. N. Data on the distribution of fibre types in five human limb muscles. An autopsy study. J Neurol Sci. 1971 Nov;14(3):245–257. doi: 10.1016/0022-510x(71)90215-2. [DOI] [PubMed] [Google Scholar]
  14. Jennekens F. G., Tomlinson B. E., Walton J. N. The sizes of the two main histochemical fibre types in five limb muscles in man. An autopsy study. J Neurol Sci. 1971 Jul;13(3):281–292. doi: 10.1016/0022-510x(71)90033-5. [DOI] [PubMed] [Google Scholar]
  15. Ludin H. P. Microelectrode study of dystrophic human skeletal muscle. Eur Neurol. 1970;3(2):116–121. doi: 10.1159/000113963. [DOI] [PubMed] [Google Scholar]
  16. Ludin H. P. Microelectrode study of normal human skeletal muscle. Eur Neurol. 1969;2(6):340–347. doi: 10.1159/000113810. [DOI] [PubMed] [Google Scholar]
  17. PADYKULA H. A., HERMAN E. The specificity of the histochemical method for adenosine triphosphatase. J Histochem Cytochem. 1955 May;3(3):170–195. doi: 10.1177/3.3.170. [DOI] [PubMed] [Google Scholar]
  18. Santa T., Engel A. G., Lambert E. G. Histometric study of neuromuscular junction ultrastructure. II. Myasthenic syndrome. Neurology. 1972 Apr;22(4):370–376. doi: 10.1212/wnl.22.4.370. [DOI] [PubMed] [Google Scholar]
  19. Santa T., Engel A. G., Lambert E. H. Histometric study of neuromuscular junction ultrastructure. I. Myasthenia gravis. Neurology. 1972 Jan;22(1):71–82. doi: 10.1212/wnl.22.1.71. [DOI] [PubMed] [Google Scholar]
  20. Santa T. Fine structure of the human skeletal muscle in myopathy. Arch Neurol. 1969 May;20(5):479–489. doi: 10.1001/archneur.1969.00480110043004. [DOI] [PubMed] [Google Scholar]
  21. Schotland D. L. An electron microscopic study of target fibers, target-like fibers and related abnormalities in human muscle. J Neuropathol Exp Neurol. 1969 Apr;28(2):214–228. [PubMed] [Google Scholar]
  22. Shafiq S. A., Gorycki M. A., Milhorat A. T. An electron microscopic study of regeneration and satellite cells in human muscle. Neurology. 1967 Jun;17(6):567–passim. doi: 10.1212/wnl.17.6.567. [DOI] [PubMed] [Google Scholar]
  23. Spurr A. R. A low-viscosity epoxy resin embedding medium for electron microscopy. J Ultrastruct Res. 1969 Jan;26(1):31–43. doi: 10.1016/s0022-5320(69)90033-1. [DOI] [PubMed] [Google Scholar]
  24. Stern L. Z., Payne C. M., Hannapel L. K. Acromegaly: histochemical and electron microscopic changes in deltoid and intercostal muscle. Neurology. 1974 Jun;24(6):589–593. doi: 10.1212/wnl.24.6.589. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Neurology, Neurosurgery, and Psychiatry are provided here courtesy of BMJ Publishing Group

RESOURCES