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. 2016 Feb 9;7(12):14125–14142. doi: 10.18632/oncotarget.7288

Figure 3. Ectopic expression of the wild-type (WT) and the mutated (M) LY75 gene in the LY75 knockdown SKOV3 clones sh-S3 and sh-S6.

Figure 3

A. The LY75 cDNA mutations are shown, which modify the target sequences for the two shRNAs used (#57363 and #57364), without altering the LY75 protein sequence. B. Representative phase-contrast images of LY75 knockdown clones sh-S3 and sh-S6 upon ectopic expression of the wild-type (WT) or the mutated (M) LY75 gene. C. Western blot analysis of the expression of LY75 and different EMT (epithelial and mesenchymal) markers in the LY75 knockdown clones sh-S3 and sh-S6 upon ectopic expression of the WT or the M LY75 gene.