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Mutation frequency summary.
| shRNA control | shRNA TopBP1 | |
|---|---|---|
| Totala | 11,210 | 17,428 |
| White | 59 | 326 |
| MF (×10-5)b | 526 | 1871 |
| Rearrangedc | 46/54 | 54/54 |
a Total number of colonies (blue + white) counted from all plates in triplicate; b MF, mutation frequency (calculated by white/total × 10-5); c rearranged/total counted, as determined by plasmid DNA that was prepared and digested with BamHI, then subjected to gel electrophoresis to highlight plasmid rearrangements. shRNA: short hairpin RNA.
