Table 3. Renal gene and circulating expression of RAS components and AGE-receptors.
Group | AT-1a gene | AT-1protein | AGE-R1 | AGE-R3 | Renin | Angiotensinogen | Ang II Plasma (pg/mL) | Ang II Cytosol (pg/mL) |
---|---|---|---|---|---|---|---|---|
WT | 1.47 ± 1.04 | 1.00 ± 0.00 | 0.97 ± 0.18 | 1.26 ± 0.91 | 1.02 ± 0.23 | 1.04 ± 0.12 | 791.7 ± 71.9 | 0.04 ± 0.06 |
WT D | 1.22 ± 0.67 | 0.83 ± 0.30 | 1.14 ± 0.17 | 1.47 ± 0.72 | 2.24 ± 2.23 | 1.34 ± 0.42* | 833.6 ± 128.0 | 0.02 ± 0.04 |
RAGE KO | 3.12 ± 1.65* | 0.41± 0.12* | 0.53 ± 0.09* | 0.72 ± 0.20 | 0.93 ± 0.26 | 1.24 ± 0.28 | 809.9 ± 125.3 | 0.07 ± 0.06 |
RAGE KO D | 2.17 ± 1.59 | 0.77 ± 0.16‡ | 0.67 ± 0.05†‡ | 1.13 ± 0.38‡ | 1.25 ± 0.44‡ | 2.07 ± 0.95‡ | 753.9 ± 56.3 | 0.09 ± 0.06† |
AT-2 KO | 5.07 ± 1.93*‡ | 0.25 ± 0.11* | 0.70 ± 0.08 *‡ | 0.95 ± 0.41 | 0.71 ± 0.23* | 0.70 ± 0.12*‡ | 251.6 ± 94.2*‡ | 0.61 ± 0.26*‡ |
AT-2 KO D | 4.13 ± 1.38† | 0.40 ± 0.32 | 0.68 ± 0.15† | 2.16 ± 0.75§|| | 0.50 ± 0.24† | 1.69 ± 0.29|| | 70.8 ± 48.6†§|| | 0.61 ± 0.35†§ |
Data presented as mean ± SD. WT – Wild Type, WT D – Wild Type Diabetic, RAGE KO – RAGE Knockout, AT-2 KO – Angiotensin II receptor type 2 Knockout, Ang II Plasma – circulating levels of angiotensin II, Ang II Cytosol – Angiotensin II levels within the renal cytosol.
p<0.05 vs WT,
p<0.05 vs WT D,
p<0.05 vs RAGE KO,
p<0.05 vs RAGE KO D,
p<0.05 vs AT-2 KO.