DEAR EDITOR,
Cisplatin (CP) as a potential chemotherapeutic drug is accompanied with nephrotoxicity[1] and hepatotoxicity.[2] Aerobic exercise could attenuate CP-induced nephrotoxicity gender dependently.[3,4] The experimental design was described before[3,4] briefly, sixty Wistar rats were divided into eight groups. The male animals in Group I (named EX + CP + EX) had aerobic exercise on a treadmill 1 h/day and five days/week for 8 weeks. Then, the exercise protocol was continued for another week which was accompanied with reducing the intensity of training, and during this week, the animals also received CP (2.5 mg/kg/day). Groups II (named EX + CP) had the same protocol as Group I without exercise in the last week during CP therapy. Groups III (named CP) and IV (named sham) received CP and saline respectively during the last week of the study without exercise. The female rats in Groups V–VIII had the same protocol as the male rats in Groups’ I–IV. The animals were exposed to moderate exercise with the 65% oxygen consumption.[5] Blood samples were obtained 1 week after CP administration.
CP increased the serum levels of aspartate aminotransferase (AST) in male (P < 0.05) not in female rats. The serum levels of AST decreased significantly in EX + CP + EX group compared to CP group (P < 0.05). The serum levels of alkaline phosphatase (ALP) decreased in all CP treated male and female groups compared to sham group (P < 0.05). The serum levels of alanine aminotransferase decreased in all male groups and EX + CP + EX and CP in female groups when compared with sham group [P < 0.05, Figure 1].
Figure 1.
The serum levels of alkaline phosphatase, alanine aminotransferase, and aspartate aminotransferase. *Indicates significant difference from negative control group (sham) and #indicates significant difference from positive control group (cisplatin) (P < 0.05) one-way ANOVA followed by the least-squares deconvolution posttest
AST increased in male positive control (Group III) compared to sham group as reported before.[6] CP increases toxicity via reduces antioxidant enzymes and increases malondialdehyde level,[6,7] and appropriate exercise increases antioxidant enzymes.[8,9] Administration of CP induces magnesium deficiency[10] whereas ALP activity was reduced due to magnesium depletion.[11] Therefore, aerobic exercise may reduce CP-induced hepatotoxicity by increasing activation of antioxidant system in male rats.
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REFERENCES
- 1.Nematbakhsh M, Ebrahimian S, Tooyserkani M, Eshraghi-Jazi F, Talebi A, Ashrafi F. Gender difference in cisplatin-induced nephrotoxicity in a rat model: Greater intensity of damage in male than female. Nephrourol Mon. 2013;5:818–21. doi: 10.5812/numonthly.10128. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 2.Pratibha R, Sameer R, Rataboli PV, Bhiwgade DA, Dhume CY. Enzymatic studies of cisplatin induced oxidative stress in hepatic tissue of rats. Eur J Pharmacol. 2006;532:290–3. doi: 10.1016/j.ejphar.2006.01.007. [DOI] [PubMed] [Google Scholar]
- 3.Noroozi J, Zeynali F, Nematbakhsh M, Pezeshki Z, Talebi A. Nonpreventive role of aerobic exercise against cisplatin-induced nephrotoxicity in female rats. Int J Prev Med. 2015;6:5. doi: 10.4103/2008-7802.160333. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 4.Zeynali F, Nematbakhsh M, Mojtahedi H, Poorshahnazari A, Talebi A, Pezeshki Z, et al. Protective role of aerobic exercise against cisplatin-induced nephrotoxicity in rats. Asian J Sports Med. 2015;6:e24901. doi: 10.5812/asjsm.24901. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 5.Powers SK, Criswell D, Lawler J, Martin D, Lieu FK, Ji LL, et al. Rigorous exercise training increases superoxide dismutase activity in ventricular myocardium. Am J Physiol. 1993;265(6 Pt 2):H2094–8. doi: 10.1152/ajpheart.1993.265.6.H2094. [DOI] [PubMed] [Google Scholar]
- 6.Mansour HH, Hafez HF, Fahmy NM. Silymarin modulates cisplatin-induced oxidative stress and hepatotoxicity in rats. J Biochem Mol Biol. 2006;39:656–61. doi: 10.5483/bmbrep.2006.39.6.656. [DOI] [PubMed] [Google Scholar]
- 7.Koc A, Duru M, Ciralik H, Akcan R, Sogut S. Protective agent, erdosteine, against cisplatin-induced hepatic oxidant injury in rats. Mol Cell Biochem. 2005;278:79–84. doi: 10.1007/s11010-005-6630-z. [DOI] [PubMed] [Google Scholar]
- 8.Shin YA, Lee JH, Song W, Jun TW. Exercise training improves the antioxidant enzyme activity with no changes of telomere length. Mech Ageing Dev. 2008;129:254–60. doi: 10.1016/j.mad.2008.01.001. [DOI] [PubMed] [Google Scholar]
- 9.Moien-Afshari F, Ghosh S, Elmi S, Rahman MM, Sallam N, Khazaei M, et al. Exercise restores endothelial function independently of weight loss or hyperglycaemic status in db/db mice. Diabetologia. 2008;51:1327–37. doi: 10.1007/s00125-008-0996-x. [DOI] [PubMed] [Google Scholar]
- 10.Lajer H, Daugaard G. Cisplatin and hypomagnesemia. Cancer Treat Rev. 1999;25:47–58. doi: 10.1053/ctrv.1999.0097. [DOI] [PubMed] [Google Scholar]
- 11.Pimstone B, Eisenberg E, Stallone W. Decrease in serum alkaline phosphatase activity produced by magnesium depletion in rats. Proc Soc Exp Biol Med. 1966;123:201–3. doi: 10.3181/00379727-123-31441. [DOI] [PubMed] [Google Scholar]