Abstract
Guinea pig and rat sciatic nerves were fixed with cacodylate-buffered aldehydes and OsO4, and were stained with ferric ion and ferrocyanide. Cytoplasmic surfaces of the non-myelinated nodal axon membrane of A-fibres display distinct electron-dense aggregates of stain. These aggregates were not observed in association with the paranodal or internodal axolemma. The membranes of C-fibres exhibit no staining under these conditions. Thus, the nodal axolemma of normal myelinated fibres is structurally distinct from both the myelinated internodal membrane, and from the axolemma of C-fibres. The ferric ion-ferrocyanide technique may provide a method for marking axonal membrane with normal nodal properties.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Karnovsky M. J. The ultrastructural basis of capillary permeability studied with peroxidase as a tracer. J Cell Biol. 1967 Oct;35(1):213–236. doi: 10.1083/jcb.35.1.213. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Landon D. N., Langley O. K. The local chemical environment of nodes of Ranvier: a study of cation binding. J Anat. 1971 Apr;108(Pt 3):419–432. [PMC free article] [PubMed] [Google Scholar]
- McDonald W. I. Pemyelination in relation to clinical lesions of the central nervous system. Br Med Bull. 1974 May;30(2):186–189. doi: 10.1093/oxfordjournals.bmb.a071192. [DOI] [PubMed] [Google Scholar]
- Quick D. C., Waxman S. G. Specific staining of the axon membrane at nodes of Ranvier with ferric ion and ferrocyanide. J Neurol Sci. 1977 Jan-Feb;31(1):1–11. doi: 10.1016/0022-510x(77)90002-8. [DOI] [PubMed] [Google Scholar]
- Rasminsky M., Sears T. A. Internodal conduction in undissected demyelinated nerve fibres. J Physiol. 1972 Dec;227(2):323–350. doi: 10.1113/jphysiol.1972.sp010035. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ritchie J. M., Rogart R. B. Density of sodium channels in mammalian myelinated nerve fibers and nature of the axonal membrane under the myelin sheath. Proc Natl Acad Sci U S A. 1977 Jan;74(1):211–215. doi: 10.1073/pnas.74.1.211. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ritchie J. M., Rogart R. B., Strichartz G. R. A new method for labelling saxitoxin and its binding to non-myelinated fibres of the rabbit vagus, lobster walking leg, and garfish olfactory nerves. J Physiol. 1976 Oct;261(2):477–494. doi: 10.1113/jphysiol.1976.sp011569. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Waxman S. G., Pappas G. D., Bennett M. V. Morphological correlates of functional differentiation of nodes of Ranvier along single fibers in the neurogenic electric organ of the knife fish Stern archus. J Cell Biol. 1972 Apr;53(1):210–224. doi: 10.1083/jcb.53.1.210. [DOI] [PMC free article] [PubMed] [Google Scholar]