Figure 3. Monocyte depletion has no effect on the induction of 11β-HSD1 activity in thioglycollate-elicited peritoneal cells.

Diphtheria toxin was administered to DTR transgenic mice 24 hours prior to the ip thioglycollate injection. Control mice were treated with thioglycollate alone. Peritoneal cells were lavaged 4 hours after the thioglycollate injection. A, May-Geimsa staining of cytocentrifuged peritoneal cells showing elicited neutrophils and monocytes from thioglycollate treated control mice (left panel), and depleted numbers of monocytes in lavages from diphtheria toxin (DT)-treated mice (right panel). B, 11β-HSD1 activity was measured as the percentage conversion of 200 nM [³H]-11-dehydrocorticosterone (11-DHC) to corticosterone in 10⁶ cells, measured after 4 and 20 hours of incubation with [³H]-11-DHC. White bars, controls; black bars, DTR transgenic mice. Photographs are representative of two different mice of each group, and values are mean ± range of conversion for two mice/group.