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. 2016 Apr 4;19:325–338. doi: 10.1007/s10456-016-9506-9

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© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 2 — Expression of CD34 in CD34⁺ and CD34⁻ PB-ECFCs fractions after magnetic separation. a CD34 mRNA levels in CD34⁻ ECFCs after cell culture in PL-EGM or CMi. Open bar depicts the CD34 mRNA levels in CD34⁻ ECFCs obtained after separation and prior the seeding in PL-EGM or CMi. Closed bars represent CD34 mRNA levels in CD34⁻ CMi and CD34⁻ PL cells after 6 days in cell culture. b Flow cytometry evaluation of the number of CD34⁺ cells in CD34⁻ ECFCs cultures expanded in CMi or PL-EGM. Data are expressed as mean ± SEM percentage of cells positive for CD34 in the cell cultures expanded either in CMi or PL-EGM (closed bars). The control CD34⁻ cells are depicted with open bar. c mRNA levels of genes associated with tip-cell phenotype in CD34⁺ and CD34⁻ PB-ECFCs obtained after magnetic separation of CD34⁻ PL. Open bar depict mRNA levels of tip-cell associated genes in CD34⁻ cells, while closed bars represent mRNA levels of same genes in CD34⁺ cells