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. 2016 Jun 8;19:373–388. doi: 10.1007/s10456-016-9513-x

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© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 5 — Anti-angiogenic activity of the combination of PEDF and VEGF on vessel formation of the chorioallantoic membrane (CAM). The same CAMs were treated with on-plants (silicone rings) containing 10 ng/ml PEDF with 25 ng/ml VEGF and control on-plants. Representative pictures of each condition immediately after application of the silicone ring on day 0 and at day 4 of the treatments are shown. After 4 days the vessel structure shows tertiary and quaternary vessels (white arrows). Addition of PEDF or VEGF alone did not affect vessel formation. Treatment with the combination of PEDF and VEGF CAM showed decreased angiogenesis by formation of fewer tertiary and quaternary vessels (white arrows)